trevigen comet analysis software (Trevigen)
86
Structured Review
Trevigen
trevigen comet analysis software
Trevigen Comet Analysis Software, supplied by Trevigen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/comet+analysis+software/analysis+comet+software+trevigen/pm42150679-89-25-25
Average 86 stars, based on 1 article reviews
Trevigen Comet Analysis Software, supplied by Trevigen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/comet+analysis+software/analysis+comet+software+trevigen/pm42150679-89-25-25
Average 86 stars, based on 1 article reviews
trevigen comet analysis software - by Bioz Stars,
2026-09
86/100 stars
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Related Articles
Software:Article Title: Targeting the COP9 signalosome overcomes platinum resistance in ovarian cancer through two distinct genome stability mechanisms Article Snippet: The medium was removed, and cells were incubated in the working medium supplemented with 0.1 μM or 1μM CSN5i-3 for two hours before treating with medium with CSN5i-3 and 50 nM trabectedin for 2 h. Following trabectedin wash-out, cells were incubated in the working medium with CSN5i-3 for 0, 2 or 4 h. The CometChip was then overlaid with 6 mL of 1% low melting agarose (Trevigen, 4250-500-02), followed by cell lysis with 50 mL lysis solution (Trevigen, 4250-500-01) for overnight at 4 °C. .. % DNA in tail was quantified with Article Title: The interaction of XPG with TFIIH through p62 and XPD is required for the completion of nucleotide excision repair Article Snippet: Following 5 J m −2 UV-C irradiation, cells were further incubated with 4 mM HU and 40 μM AraC at 37°C for 0, 1, 2, and 4 h. CometChip gels were then covered with 1% LM Agarose (Trevigen, 4250-050-02) and incubated with lysis buffer (Trevigen, 4250-050-01) overnight at 4°C. .. Images were captured on a fluorescence microscope (Olympus, BX53), and the percentage of DNA in the tail was quantified using Article Title: Combining Multiplexed CRISPR/Cas9-Nickase and PARP Inhibitors Efficiently and Precisely Targets Cancer Cells Article Snippet: DNA in the CometSlide was stained for 2 h at room temperature with SYBRTM Gold Nucleic Acid Gel Stain (Invitrogen, #S11494) and then visualized under a fluorescence microscope (Olympus, BX53). .. The tail moment was quantified using Article Title: Collateral damage of NUDT15 deficiency in cancer provides a cancer pharmacogenetic therapeutic window with thiopurines Article Snippet: The cells were imaged using the Carl Zeiss LSM Confocal Microscope camera using Zen Pro image processing system at a 10x magnification. .. The images were then scored using Article Title: Utilization of the CometChip assay for detecting PAH-induced DNA bulky adducts in a 3D primary human bronchial epithelial cell model Article Snippet: Chips were imaged by a Keyence BZ-X710 Fluorescence Microscope (Keyence, Itasca, IL) using a Nikon Plan Fluor 10x/0.30∞/1.2 WD 15.2 objective lens (Keyence, Itasca, IL) taking 81 images per macro-well and using Image Merge BZ-XAnalyzer to stitch images into one image per macro-well. .. CometChip images were loaded into the Trevigen other:Article Title: STK19 facilitates the clearance of lesion-stalled RNAPII during transcription-coupled DNA repair. Article Snippet: DNA fragment was cloned into the 1C cloning vector (addgene no. 29654) by ligation-independent cloning.86 Components of the RNAPII-TCR complex were cloned, expressed using Sf9, Sf21 and Hi5 insect cells (ThermoFisher, catalog no. 12659017, Expression Systems, catalogue no. 94- 003F and Expression Systems, catalogue no. 94-002F, respectively) and purified as previously described.12,43,65 Human STK19 was expressed in E. coli BL21 DE3 cells in LB media. Article Title: Combining Multiplexed CRISPR/Cas9-Nickase and PARP Inhibitors Efficiently and Precisely Targets Cancer Cells Article Snippet: After solidification of the agarose, the slides were immersed in prechilled lysis solution (R&D Systems, #4250-050-01) and incubated overnight at 4°C. Article Title: STK19 facilitates the clearance of lesion-stalled RNAPII during transcription-coupled DNA repair. Article Snippet: Babraham Bioinformatics https://www.bioinformatics.babraham.ac. uk/projects/trim_galore/ bwa-mem tools (BWA, Version 0.7.17) Li74 GitHub - lh3/bwa: Burrow-Wheeler Aligner for short-read alignment (see minimap2 for longread alignment) Samtools (Version 1.11) Danecek et al.75 https://github.com/samtools/samtools HOMER tools (Version 4.8.2) Heinz et al.76 http://homer.ucsd.edu./homer/ IGV (Version 2.4.3) Robinson et al.77 https://igv.org STAR (Version 2.5.2a and 2.7.7a) Dobin et al.78 https://github.com/alexdobin/STAR R (Version 4.0.5) R Core Team79 N/A Rstudio (Version 1.1.423) RStudio Team, 2020 http://www.rstudio.com/ Cutadapt (Version 1.18) Martin80 https://github.com/marcelm/cutadapt/ DESeq2 Love et al.81 https://github.com/thelovelab/DESeq2 Deeptools (Version 2.5.3) Ramirez et al.82 https://github.com/deeptools/deepTools BEDTools (Version 2.27.1) Quinlan and Hall83 https://github.com/arq5x/bedtools2 ll OPEN ACCESS e5 Cell 187, 1–19.e1–e13, December 12, 2024 Please cite this article in press as: van den Heuvel et al., STK19 facilitates the clearance of lesion-stalled RNAPII during transcription-coupled DNA repair, Cell (2024), https://doi.org/10.1016/j.cell.2024.10.018 Article ll OPEN ACCESS Please cite this article in press as: van den Heuvel et al., STK19 facilitates the clearance of lesion-stalled RNAPII during transcription-coupled DNA repair, Cell (2024), https://doi.org/10.1016/j.cell.2024.10.018 Fluorescence:Article Title: The interaction of XPG with TFIIH through p62 and XPD is required for the completion of nucleotide excision repair Article Snippet: Following 5 J m −2 UV-C irradiation, cells were further incubated with 4 mM HU and 40 μM AraC at 37°C for 0, 1, 2, and 4 h. CometChip gels were then covered with 1% LM Agarose (Trevigen, 4250-050-02) and incubated with lysis buffer (Trevigen, 4250-050-01) overnight at 4°C. .. Images were captured on a fluorescence microscope (Olympus, BX53), and the percentage of DNA in the tail was quantified using Microscopy:Article Title: The interaction of XPG with TFIIH through p62 and XPD is required for the completion of nucleotide excision repair Article Snippet: Following 5 J m −2 UV-C irradiation, cells were further incubated with 4 mM HU and 40 μM AraC at 37°C for 0, 1, 2, and 4 h. CometChip gels were then covered with 1% LM Agarose (Trevigen, 4250-050-02) and incubated with lysis buffer (Trevigen, 4250-050-01) overnight at 4°C. .. Images were captured on a fluorescence microscope (Olympus, BX53), and the percentage of DNA in the tail was quantified using |